soft substrate 12 kpa Search Results


cgas-mcherry  (CH Instruments)
90
CH Instruments cgas-mcherry
Cgas Mcherry, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+substrate+12+kpa/cgas+mcherry/pmc10257411__MOL2___17___1007___s002-39-236-249
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medical grade soft silicone sheets  (Verlag GmbH)
90
Verlag GmbH medical grade soft silicone sheets
Medical Grade Soft Silicone Sheets, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kpa rigid substrates  (Matrigen Life Technologies)
94
Matrigen Life Technologies kpa rigid substrates
BMP4-induced EMT is suppressed on compliant <t>substrates.</t> a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001
Kpa Rigid Substrates, supplied by Matrigen Life Technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+substrate+12+kpa/Softslip+12/pmc07018387-357-12-17
Average 94 stars, based on 1 article reviews
kpa rigid substrates - by Bioz Stars, 2026-09
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organic-matter rich substrate  (Biomix Inc)
90
Biomix Inc organic-matter rich substrate
BMP4-induced EMT is suppressed on compliant <t>substrates.</t> a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001
Organic Matter Rich Substrate, supplied by Biomix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+substrate+12+kpa/commercial+substrate+biomix/pm35830071-47-12-15
Average 90 stars, based on 1 article reviews
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nano-glo® in vivo substrate cs320501  (Promega)
90
Promega nano-glo® in vivo substrate cs320501
BMP4-induced EMT is suppressed on compliant <t>substrates.</t> a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001
Nano Glo® In Vivo Substrate Cs320501, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+substrate+12+kpa/fluorofurimazine/pmc10372078-289-5-12
Average 90 stars, based on 1 article reviews
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chemiluminescent substate  (Bio-Rad)
99
Bio-Rad chemiluminescent substate
BMP4-induced EMT is suppressed on compliant <t>substrates.</t> a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001
Chemiluminescent Substate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+substrate+12+kpa/Clarity+Western+ECL+Substrate/pmc11829800-425-6-12
Average 99 stars, based on 1 article reviews
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westernsure premium chemiluminescent substract  (LI-COR)
96
LI-COR westernsure premium chemiluminescent substract
BMP4-induced EMT is suppressed on compliant <t>substrates.</t> a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001
Westernsure Premium Chemiluminescent Substract, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+substrate+12+kpa/WESTERNSURE+PREMIUM+CHEMI+SUBSTRATE/pmc06075618-420-4-8
Average 96 stars, based on 1 article reviews
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clarity max western ecl substarte  (Bio-Rad)
97
Bio-Rad clarity max western ecl substarte
BMP4-induced EMT is suppressed on compliant <t>substrates.</t> a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001
Clarity Max Western Ecl Substarte, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+substrate+12+kpa/Clarity+Max+Western+ECL+Substrate/pm38167707-253-36-41
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chemiluminescence  (Bio-Rad)
99
Bio-Rad chemiluminescence
BMP4-induced EMT is suppressed on compliant <t>substrates.</t> a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001
Chemiluminescence, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+substrate+12+kpa/Clarity+ECL+Western+Substrate/pm40712754-99-10-18
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signalstain dab substate kit  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc signalstain dab substate kit
BMP4-induced EMT is suppressed on compliant <t>substrates.</t> a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001
Signalstain Dab Substate Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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dab substrate reagent from peroxidase substract kit  (Vector Laboratories)
96
Vector Laboratories dab substrate reagent from peroxidase substract kit
BMP4-induced EMT is suppressed on compliant <t>substrates.</t> a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001
Dab Substrate Reagent From Peroxidase Substract Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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substrate from kit  (Novozymes limited)
90
Novozymes limited substrate from kit
BMP4-induced EMT is suppressed on compliant <t>substrates.</t> a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001
Substrate From Kit, supplied by Novozymes limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


BMP4-induced EMT is suppressed on compliant substrates. a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001

Journal: Oncogene

Article Title: Mediator kinase CDK8/CDK19 drives YAP1-dependent BMP4-induced EMT in cancer

doi: 10.1038/s41388-018-0316-y

Figure Lengend Snippet: BMP4-induced EMT is suppressed on compliant substrates. a Phase contrast images of cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels and treated with 10 nM BMP4 for up to 5 days to monitor any changes in morphology (Methods). b Immunofluorescence images of BMP4-treated OvCa429 and Panc1 cells plated on soft (0.5 kPa) and rigid (8 kPa) fibronectin-coated hydrogels as described in Methods. Cells were then immunostained for E-cadherin and phalloidin/actin as indicated. Scale bars = 20 μm. c Realtime quantitative PCR of SNAI1 and SNAI2 in OvCa429, Panc1, and Py2T cells plated on soft (0.5 kPa) and rigid (8 kPa) hydrogels followed by treatment with 10 nM BMP4 (Methods). Values are normalized to their untreated control. Graphs are representative of two independent biological trials done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: For rigidity experiments, cells were plated on 0.5 kPa (soft) or 8 kPa (rigid) substrates obtained from Matrigen Life Technologies (Brea, CA, USA), (Softslip 12-well and Softwell 6-well) that were coated with 10 μg/mL fibronectin (Cultrex® #3420–001-01) in 1 × PBS for 1 h. All cells were allowed to sit on substrates for at least 12 h before BMP4 treatment.

Techniques: Immunofluorescence, Real-time Polymerase Chain Reaction, Control